Melastatin expression determined by chromogenic in situ hybridization (CISH) in primary cutaneous melanoma

Autor:	Lynn A. Cornelius, T. M. Nazir, G. Bowers, J.S. Ross, A. Dejulio, Q. Li, J. A. Carlson, Christine E. Sheehan, Martin C. Mihm, Gerald P. Linette
Rok vydání:	2004
Předmět:	Oncology Cancer Research medicine.medical_specialty Univariate analysis Pathology Formalin fixed paraffin embedded business.industry Melanoma Chromogenic in situ hybridization In situ hybridization medicine.disease Internal medicine Localized disease Cutaneous melanoma Medicine business CISH
Zdroj:	Journal of Clinical Oncology. 22:7517-7517
ISSN:	1527-7755 0732-183X
DOI:	10.1200/jco.2004.22.90140.7517
Popis:	7517 Background: Using a radioactive in situ hybridization method on primary melanoma (PM), loss of the melastatin (MLSN-1) mRNA expression has been found to have independent prognostic significance. In this study, we examined whether a new non-radioactive chromogenic ISH (CISH) method to evaluate melastatin expression would similarly predict PM outcome. Methods: Melastatin mRNA was evaluated by CISH on formalin fixed paraffin embedded tissues from 109 PM patients with localized disease (AJCC stages I and II). PMs were classified as having complete or partial down regulation of MLSN compared to internal control intraepidermal melanocytes. MLSN status was compared with standard prognostic factors over a mean clinical follow-up of 58 months with a range of 2 to 225 months. Results: On univariate analysis, partial or complete loss of MLSN expression, found in 32% and 27% of PM respectively, correlated with disease free survival (DFS) (p
Databáze:	OpenAIRE
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