Determination of Cefazolin in Human Serum by High Performance Liquid Chromatography with On-Line Solid Phase Extraction
| Autor: | Fp. Alo, L. Leone, G. Ioannidis, S. Bompadre, L. Ferrante |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Chromatography
Chemistry Clinical Biochemistry Extraction (chemistry) Cefazolin Pharmaceutical Science Biochemistry High-performance liquid chromatography Analytical Chemistry Blood serum Pharmacokinetics medicine Solid phase extraction Quantitative analysis (chemistry) Antibacterial agent medicine.drug |
| Zdroj: | Journal of Liquid Chromatography & Related Technologies. 21:417-426 |
| ISSN: | 1520-572X 1082-6076 |
| DOI: | 10.1080/10826079808000500 |
| Popis: | A simple, sensitive, reproducible, and rapid high performance liquid chromatographic assay, with column switching, is described for the determination of cefazolin in human serum. Serum samples were directly injected, first, in a chromatographic column for sample clean-up and extraction, and then, analysed on a analytical column using ultraviolet absorption at 273 nm. The assay allows direct determination of cefazolin over the range of 1 - 250 μg/mL using 12.5 μL of serum. Validation of the method demonstrated a good sensitivity, accuracy, and precision. It has been applied to study the pharmacokinetics of the drug in patients undergoing vascular surgery. |
| Databáze: | OpenAIRE |
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