Determination and pharmacokinetic study of jaceosidin in rat plasma by UPLC–MS/MS
| Autor: | Xianqin Wang, Xiaomin Yu, Congcong Wen, Shuanghu Wang, Bingbao Chen, Junyan Chen, Yanwen Dong, Yunfang Zhou |
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| Rok vydání: | 2018 |
| Předmět: |
Avicularin
Chromatography 010405 organic chemistry Formic acid Electrospray ionization 010401 analytical chemistry Selected reaction monitoring Analytical chemistry General Chemistry Mass spectrometry 01 natural sciences High-performance liquid chromatography 0104 chemical sciences chemistry.chemical_compound chemistry Protein precipitation Acetonitrile |
| Zdroj: | Acta Chromatographica. 30:131-135 |
| ISSN: | 2083-5736 |
| DOI: | 10.1556/1326.2017.00104 |
| Popis: | In this work, a sensitive and selective ultra-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) method was developed and fully validated for determination of jaceosidin in rat plasma. Avicularin was used as the internal standard (IS), and protein precipitation by acetonitrile was used to prepare samples. Chromatographic separation was achieved on a UPLC BEH C18 column (2.1 mm × 100 mm, 1.7 μm) with 0.1% formic acid and acetonitrile as the mobile phase with gradient elution. An electrospray ionization (ESI) source was applied and operated in positive ion mode; multiple reaction monitoring (MRM) mode was used for quantification. Calibration plots were linear throughout the range 2–500 ng mL−1 for jaceosidin in rat plasma. Relative standard deviation (RSD) of intra-day and inter-day precision was less than 12%. The accuracy of the method was between 88.7% and 109.7%. Mean recoveries of jaceosidin in rat plasma ranged from 65.4% to 77.9%. The developed UPLC–MS/MS method was successfully ... |
| Databáze: | OpenAIRE |
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