Using Protein Chips to Develop a Highly Specific HER2 Antibody for HER2 Amplification Testing

Autor: Guangli Wang, Zhongwu Li, Lixin Zhou, Ma Donghui, Min Zhao, Shu Youmin, Kehu Yuan, Wei Fu, Jian Chen, Qi Lili, Boyang Chu, Huibo Liu, Guiyin Wu, He Weiwu, Xiaozheng Huang, Yi Shen
Rok vydání: 2015
Předmět:
Zdroj: The FASEB Journal. 29
ISSN: 1530-6860
0892-6638
Popis: Human epidermal growth factor receptor 2 (HER2) is an orphan receptor tyrosine kinase member of the EGFR families and is found to be a key tumor driver gene. In breast cancer and gastric cancer, HER2 amplification can be effectively treated by its neutralizing antibody, Herceptin. In clinic, the HER2 immunohistochemistry (IHC) was used as the primary screening method to diagnose HER2 amplification. However, recent evidence suggested that the frequently used rabbit HER2 antibody 4B5 cross reacted to another family member HER4. IHC staining also indicated that it has strong non-specific cytoplasmic and nucleus staining in normal gastric mucosal cells and some gastric cancer samples. Using a protein lysate array which covers 85% of the human proteome, we have successfully identified and confirmed that the 4B5 bound to HER4 and a nuclear protein ZSCAN18 besides HER2. The non-specific binding accounts for the unexpected cytoplasmic and unclear staining of 4B5 on normal gastric epithelium. Finally, we have deve...
Databáze: OpenAIRE
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