Notch signaling pathway in cumulus cells reflecting zygote and embryo quality in polycystic ovary syndrome
| Autor: | Farid Abolhassani, Azadeh Hosseini Najarkolaei, Ebrahim Asadi, Nazila Yamini, Fahimeh Salehi, Ashraf Moini, Sahar Esfandiyari, Maryam Azizi Kutenaee, Reza Aflatoonian, Elham Hosseini, Mojtaba Masoudi, Keywan Mortezaee, Ensieh Salehi |
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| Rok vydání: | 2021 |
| Předmět: |
endocrine system
education.field_of_study 030219 obstetrics & reproductive medicine Zygote business.industry medicine.medical_treatment Population Notch signaling pathway Obstetrics and Gynecology Embryo General Medicine Polycystic ovary Intracytoplasmic sperm injection Andrology 03 medical and health sciences 0302 clinical medicine 030220 oncology & carcinogenesis medicine education business Receptor Embryo quality |
| Zdroj: | Archives of Gynecology and Obstetrics. 304:1097-1105 |
| ISSN: | 1432-0711 0932-0067 |
| Popis: | The present study aimed to explore the associations between the expression pattern of molecules in the Notch pathway in the cumulus cells of polycystic ovary syndrome (PCOS) patients and the quality of zygotes and embryos. A total of 200 cumulus complexes surrounding mature oocytes were obtained from 40 patients with and without PCOS undergoing intracytoplasmic sperm injection (ICSI). The expressions of Notch-1, Notch-2, and Notch-3 genes were examined by Reverse Transcription Q-PCR assay. Moreover, immunocytochemistry was performed for the expressions of Jagged-1 and Jagged-2 proteins. The correlations between the Notch receptors and their ligand expressions and the qualities of the zygote and embryo were investigated. The expression levels of Notch-2, Notch-3, Jagged-1, and Jagged-2 were significantly lower in patients with PCOS than in normal women (p |
| Databáze: | OpenAIRE |
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