In vivo multiphoton microscopy beyond 1 mm in the brain
| Autor: | Evan P. Perillo, S. M. Shams Kazmi, Kristen Hagan, Ahmed M. Hassan, Andrew K. Dunn, David R. Miller, Flor A. Medina, Boris V. Zemelman |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Fluorescence-lifetime imaging microscopy Materials science Neocortex business.industry 01 natural sciences Optical parametric amplifier 010309 optics 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology Optics Multiphoton fluorescence microscope medicine.anatomical_structure Two-photon excitation microscopy chemistry In vivo 0103 physical sciences medicine Fluorescence microscope business Indocyanine green Biomedical engineering |
| Zdroj: | SPIE Proceedings. |
| ISSN: | 0277-786X |
| DOI: | 10.1117/12.2249949 |
| Popis: | We perform high-resolution, non-invasive, in vivo deep-tissue imaging of the mouse neocortex using multiphoton microscopy with a high repetition rate optical parametric amplifier laser source tunable between λ=1,100 and 1,400 nm. We demonstrate an imaging depth of 1,200 μm in vasculature and 1,160 μm in neurons. We also demonstrate deep-tissue imaging using Indocyanine Green (ICG), which is FDA approved and a promising route to translate multiphoton microscopy to human applications. |
| Databáze: | OpenAIRE |
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