The glycosyltransferase UGT76B1 is critical for plant immunity as it governs the homeostasis of N-hydroxy-pipecolic acid
| Autor: | Lennart Mohnike, Yuelin Zhang, Dmitrij Rekhter, Weijie Huang, Kirstin Feussner, Ivo Feussner, Cornelia Herrfurth, Hainan Tian |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0303 health sciences Innate immune system biology Mutant Plant Immunity 01 natural sciences humanities Cell biology 03 medical and health sciences chemistry.chemical_compound Immune system chemistry Glycosyltransferase Plant defense against herbivory biology.protein Systemic acquired resistance 030304 developmental biology 010606 plant biology & botany Pipecolic acid |
| DOI: | 10.1101/2020.06.30.179960 |
| Popis: | The trade-off between growth and defense is a critical aspect of plant immunity. Therefore, plant immune response needs to be tightly regulated. The hormone regulating plant defense against biotrophic pathogens is salicylic acid (SA). Recently, N-hydroxy-pipecolic acid (NHP) was identified as second regulator for plant innate immunity and systemic acquired resistance. Although the biosynthetic pathway leading to NHP formation has already been identified, the route how NHP is further metabolized was unclear. Here, we present UGT76B1 as a UDP-dependent glycosyltransferase that modifies NHP by catalyzing the formation of 1-O-glucosyl-pipecolic acid (NHP-OGlc). Analysis of T-DNA and CRISPR knock-out mutant lines of UGT76B1 by targeted and non-targeted UHPLC-HRMS underlined NHP and SA as endogenous substrates of this enzyme in response to Pseudomonas infection and UV treatment. UGT76B1 shows similar KM for NHP and SA. ugt76b1 mutant plants have a dwarf phenotype and a constitutive defense response which can be suppressed by loss of function of the NHP biosynthetic enzyme FMO1. This suggests that elevated accumulation of NHP contributes to the enhanced disease resistance in ugt76b1. Externally applied NHP can move to distal tissue in ugt76b1 mutant plants. Although glycosylation is not required for the long distance movement of NHP during systemic acquired resistance, it is crucial to balance growth and defense. |
| Databáze: | OpenAIRE |
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