Role of fatty acid salts as anti Acanthamoeba agents for disinfecting contact lens
| Autor: | Yumeho Obata, Hiroshi Morita, Aya Tanaka, Takayoshi Kawahara, Mariko Era, Manami Masuda, Takahide Kanyama |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
chemistry.chemical_classification Chromatography biology 030106 microbiology Public Health Environmental and Occupational Health Fatty acid Butyrate medicine.disease biology.organism_classification Applied Microbiology and Biotechnology Microbiology Acanthamoeba Contact lens 03 medical and health sciences Minimum inhibitory concentration 0302 clinical medicine Acanthamoeba keratitis chemistry parasitic diseases 030221 ophthalmology & optometry medicine Acanthamoeba castellanii Linolenate |
| Zdroj: | Biocontrol Science. 22:153-161 |
| ISSN: | 1884-0205 1342-4815 |
| Popis: | Acanthamoeba is found in seawater, fresh water, and soil and is an opportunistic pathogen that causes a potentially blinding corneal infection known as Acanthamoeba keratitis. The anti-amoeba activity of 9 fatty acid salts (potassium butyrate (C4K), caproate (C6K), caprylate (C8K), caprate (C10K), laurate (C12K), myristate (C14K), oleate (C18:1K), linoleate (C18:2K), and linolenate (C18:3K)) was tested on Acanthamoeba castellanii ATCC 30010 (trophozoites and cysts). Fatty acid salts (350 mM and pH 10.5) were prepared by mixing fatty acids with the appropriate amount of KOH. C8K, C10K, and C12K showed growth reduction of 4 log-units (99.99% suppression) in A. castellanii upon 180 min incubation at 175 mM, whereas the pH-adjusted control solution showed no effect. After the amoeba suspension was mixed with C10K or C12K, cell membrane destruction was observed. The minimum inhibitory concentration of C10K and C12K was also determined to be 2.7 mM. Confirmation tests were conducted using contact lenses to evaluate the effectiveness of C10K and C12K as multi-purpose solutions. Experiments using increasing concentrations showed reduced numbers of living cells in C10K (5.5 mM, 10.9 mM) and in C12K (5.5 mM, 10.9 mM). These results demonstrate the inhibitory activity of C10K and C12K against A. castellanii and indicate their potential as anti-amoeba agents. |
| Databáze: | OpenAIRE |
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