Rapid and Sensitive Method to Detect Oxidized Forms of rhGCSF Using Agilent 2100 Bioanalyzer

Autor:	Sampali Banerjee, Sandeep Somani, Ketaki Sabnis Prasad, Goutam Mandal, Sriram Padmanabhan
Rok vydání:	2009
Předmět:	Methionine Biochemistry (medical) Clinical Biochemistry Cell Proliferation assay Sulfoxide Biological activity Biochemistry Dithiothreitol Analytical Chemistry Granulocyte colony-stimulating factor chemistry.chemical_compound medicine.anatomical_structure chemistry Electrochemistry medicine Hydrogen peroxide Spectroscopy
Zdroj:	Analytical Letters. 42:1070-1083
ISSN:	1532-236X 0003-2719
DOI:	10.1080/00032710902890454
Popis:	Oxidation of methionine of human granulocyte colony stimulating factor (GCSF) results in loss of biological activity. In this study, we report the use of an Agilent 2100 bioanalyzer to detect oxidized forms of rhGCSF after exposure to hydrogen peroxide (H2O2). Our data show that the bioanalyzer is capable of detecting minor changes in rhGCSF after oxidation with 0.01% (w/v) H2O2, which results in nearly 50% loss in biological activity as observed by cell (NFS-60) proliferation assay. Dithiothreitol could largely protect such a H2O2-mediated oxidation, and thus, we conclude that the major modification of GCSF upon methionine oxidation is the conversion of methionines to its sulfoxide.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::76761d6d21ef9194014a8b4b79531fd9 https://doi.org/10.1080/00032710902890454 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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