TheAgrobacteriumDNA Transfer Complex
| Autor: | John Zupan, Patricia Zambryski, Vitaly Citovsky |
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| Rok vydání: | 1997 |
| Předmět: | |
| Zdroj: | Critical Reviews in Plant Sciences. 16:279-295 |
| ISSN: | 1549-7836 0735-2689 |
| DOI: | 10.1080/07352689709701951 |
| Popis: | Agrobacterium tumefaciens elicits tumorous growths on plants by transforming plant cells with a segment of its own DNA. This trait led to development of Agrobacterium as a vector for genetic transformation of flowering plants. The transformation process is a unique mixture of several distinct steps, some of which are evolutionarily and functionally related to bacterial conjugation, and some of which converge with eukaryotic cellular processes. Recent work has advanced our understanding of each of these steps. The early reactions in the production of an ssDNA transfer intermediate (T-strand), mediated by the VirD1/D2 proteins, are chemically similar to formation of a relaxosome in bacterial conjugation. The T-strand is coated by the ssDNA binding protein VirE2; however, whether this binding occurs in the bacterium or in planta is disputed. VirB proteins, related to proteins for the conjugal transfer of DNA between bacteria, most likely form the transfer apparatus. VirD2, which remains covalently b... |
| Databáze: | OpenAIRE |
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