| Popis: |
After a single skin surface dose of 3000 rep of β-irradiation from a ${}^{90}{\rm Sr}-{}^{90}{\rm Y}$ sealed source, epidermis of albino guinea pigs was excised and washed in 0.9% NaCl ("extracellular" fraction), and the washed tissue was homogenized in saline (intracellular fraction). The time course of changes in acid phosphatase, alkaline 5′-nucleotidase (AMPase), and inorganic pyrophosphatase was followed. The earliest increase in intracellular acid phosphatase was detected about 6 days postirradiation, after pyknosis. Histochemical localization revealed minute acid phosphatase-rich granules primarily in the cells of the stratum granulosum. These increased in size and number in the cells of the hyperplastic epidermis 30 days postirradiation when the peak increases in "extra"- and intracellular acid phosphatase occurred. Treatment with Triton X-100 resulted in diffuse staining without granule formation, suggesting that these granules may be lysosomes. There was a progressive decrease in "extra"- and in... |
