| Popis: |
The amino acid composition and terminal amino acids of two typical individual specimens of Bence-Jones proteins were determined. The amino acid composition of the specimens was very similar, and it was noteworthy with its high hydroxyamino acid content. Subfractionation of one of the specimens was accomplished by the diethylaminoethyl ion exchange chromatography. It was also found that the specimens contained half-cystine, in addition to aspartic acid, as the N-terminal group. The halfcystine, however, was present only in one of the subfractions, while the others were devoid of it. The proteins showed the characteristic propanol flocculation behavior which can be used for their testing in urine for diagnostic purposes. |
