Induction of corneal epithelial progenitors from bone-marrow mesenchymal stem cells of rhesus monkeys in vitro
| Autor: | Bing Huang, JianXiong Yu, Jian Ge, Jingbo Liu, Bingqian Liu, Ruzhang Jiang, Jing Yuan |
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| Rok vydání: | 2007 |
| Předmět: |
Multidisciplinary
medicine.diagnostic_test Growth factor medicine.medical_treatment Mesenchymal stem cell Biology eye diseases Bone marrow mesenchymal stem cells In vitro Flow cytometry Cell biology Immunology medicine sense organs PAX6 Progenitor cell Stem cell transplantation for articular cartilage repair |
| Zdroj: | Chinese Science Bulletin. 52:2216-2225 |
| ISSN: | 1861-9541 1001-6538 |
| DOI: | 10.1007/s11434-007-0304-z |
| Popis: | Bioengineered corneas are substitutes for human donor tissue that are designed to treat severe disease affecting ocular surfaces. However, a shortage of candidate seed cells for bioengineering corneas is still a problem. Bone-marrow mesenchymal stem cells (MSCs) are capable of multilineage differentiation. Therefore, we determined whether MSCs differentiate into corneal epithelial cells (ECs). We applied three exoteric-microenvironmental systems to induce MSCs to become ECs. Induced MSC were identified by means of morphologic examination, immunocytochemical analysis, and flow cytometry. MSCs grown in one microenvironment had characteristics similar to those of corneal epithelial progenitors. Induced MSCs expressed markers for EC, including integrin β1, Cx43, Pax6, and P63. MSCs were successfully induced to become corneal epithelial progenitors. Therefore, the use of MSCs may hold substantial promise for reconstructing the ocular surface after corneal injury. |
| Databáze: | OpenAIRE |
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