A time-resolved fluorescence immunosensing platform for highly sensitive detection of cardiac troponin I
| Autor: | Yong Duk Han, Ka Ram Kim, Kook-Nyung Lee, D. K. Hong, Kwan Woo Lee, Hyun C. Yoon, Hyeong Jin Chun |
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| Rok vydání: | 2017 |
| Předmět: |
Chromatography
Materials science Cardiac troponin medicine.diagnostic_test Nanotechnology 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences Fluorescence 0104 chemical sciences Highly sensitive Silica nanoparticles Immunoassay Troponin I medicine Time-resolved spectroscopy 0210 nano-technology |
| Zdroj: | 2017 19th International Conference on Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS). |
| DOI: | 10.1109/transducers.2017.7994378 |
| Popis: | In this study, we developed a novel time-resolved fluorescence (TRF)-based immunosensing platform that consisted with the physically stable europium chelate-encapsulated silica nanoparticle and the simplified TRF analyzer. To verify the applicability of developed TRF immunosensing platform toward practical immunoassay, the sandwich immunosensing of cardiac troponin I (cTnI) was accomplished using the synthesized europium-encapsulated silica nanoparticles as a signaling probe and homemade TRF analysis system as an optical transducer. |
| Databáze: | OpenAIRE |
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