| Popis: |
The phytochemicals can act as reducing agents as well as nontoxic capping materials of metal nanosilver. The objective of the present work was to fabricate the synthesis of silver nanoparticle using aqueous bark extract of Saraca asoca as reducing agent and testing the wound healing efficacy of the material by studying the level of wound contraction and re-epithelialization and expression of inflammatory factors like cytokines and different proinflammatory markers in diabetic mice. The biocompatibility of the synthesized silver nanoparticle was tested by cell cycle analysis, ROS generation, DAPI, comet assay. It was indicated that the synthesized silver nanoparticles were nontoxic to normal mice in vivo. Antibacterial properties of the silver formulation are comparable to different antibiotics and more effective against Bacillus firmus and Staphylococcus gallinarum of diabetic wound. SEM images and histopathological observations revealed that the healing process was accelerated in the silver nanoparticle treated group. The significant healing efficacy of phytofabricated nanoparticles was evident by the observations of downregulation of proinflammatory cytokines such as IL-1β, IL-6, and TNF-α, and upregulation of anti-inflammatory cytokine, IL-10. |
