Serum levels of inflammatory biomarkers, glycaemic control indices and leptin receptors expression in adult male Wistar rats exposed to Pyrethroids
Autor: | H.B. Osadolor, Adebimpe Iyanuoluwa Moronkeji, Akinpelu Moronkeji, Adedeji David Atere |
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Rok vydání: | 2021 |
Předmět: |
0301 basic medicine
medicine.medical_specialty Leptin receptor Adult male business.industry 030209 endocrinology & metabolism Applied Microbiology and Biotechnology Inflammatory biomarkers 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Endocrinology Internal medicine Medicine business Molecular Biology Biotechnology |
Zdroj: | Journal of Cellular Biotechnology. 7:41-55 |
ISSN: | 2352-3697 2352-3689 |
Popis: | BACKGROUND: Due to widespread use of pesticides, an individual’s lifetime exposure to pesticide mixtures is unavoidable, resulting in potentiation of toxic effects such as changing metabolic signaling temporarily or permanently due to the formation of free radicals among exposed individuals. OBJECTIVE: This study then looked at the expression of leptin and insulin receptors in Wistar rats exposed to pyrethroid and its effects on adipokines and glycemic indices. METHODS: 84 Wistar rats were randomly selected and divided into two groups. 51 rats were exposed to 1.2 % w/v pyrethroid insecticides, while the remaining 33 rats were grouped as non-exposed. The groups were divided into three different groups, each with 7, 21, and 41 days. The animals were anesthetically sacrificed and samples of blood were collected after days of exposure. The levels of total antioxidant status (TAS), malondialdehyde (MDA), glutathione (GSH), hydrogen peroxide (H2O2), nitric oxide (NO), glucose and lipids were determined. Blood activities of superoxide dismutase (SOD), glutathione peroxidase (GPX), and catalase (CAT) were also determined. ELISA was used to assess leptin, adiponectin, insulin, CRP, IL-6, and TNFα. Glycaemic indices were calculated appropriately. Leptin and Insulin expression was determined using the reverse transcriptase-polymerase chain reaction (RT-PCR) method. Data obtained were statistically compared, and P≤0.05 was considered significant. RESULTS: The mean TChol, TAG, and HDL-C were significantly different (p |
Databáze: | OpenAIRE |
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