Characterization of Colibactin-Producing Escherichia coli Isolated from Japanese Patients with Colorectal Cancer
| Autor: | Fumi Higashiguchi, Hideki Ishikawa, Yuji Iwashita, Haruhiko Sugimura, Yuichiro Hirayama, Kenji Watanabe, Noriyuki Miyoshi, Nobuo Matsuzaki, Michihiro Mutoh, Yuta Tsunematsu, Keiji Wakabayashi, Yuko Yoshikawa, Michio Sato |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Microbiology (medical) biology Colorectal cancer 030106 microbiology General Medicine medicine.disease_cause medicine.disease biology.organism_classification Molecular biology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Infectious Diseases Biosynthesis chemistry Colibactin medicine Selective advantage Colorectal adenocarcinoma 030212 general & internal medicine Carcinogenesis Escherichia coli Bacteria |
| Zdroj: | Japanese Journal of Infectious Diseases. 73:437-442 |
| ISSN: | 1884-2836 1344-6304 |
| DOI: | 10.7883/yoken.jjid.2020.066 |
| Popis: | We investigated the relationship between colibactin-producing (clb+) Escherichia coli and colorectal adenocarcinoma. In total, 729 E. coli colonies were isolated from tumor and surrounding non-tumor regions in resected specimens from 34 Japanese patients; 450 colonies were from the tumor regions and 279 from the non-tumor regions. clb+ bacteria were found in tumor regions of 11 patients (11/34, 32.4%) and they were also detected in the non-tumor regions of 7 out of these 11 patients (7/34, 20.6%). The prevalence of clb+ isolates was 72.7% (327/450) and 44.1% (123/279) in tumor and non-tumor regions, respectively. All the recovered clb+ isolates belonged to the phylogenetic group B2 and were the most predominant type in tumor regions. Hemolytic (α-hemolysin-positive, hlyA+) and non-hemolytic (α-hemolysin-negative, hlyA-) clb+ isolates were obtained from patient #19; however, the prevalence of hlyA+ clb+ isolates was significantly higher in tumor regions (35/43, 81.4%) than in non-tumor regions (3/19, 15.8%). Moreover, a significantly higher production of N-myristoyl-D-asparagine, a by-product of colibactin biosynthesis, was observed in hlyA+ clb+ isolates than in hlyA- clb+ isolates. Our results suggest that hlyA+ clb+ E. coli may have a selective advantage in colorectal colonization and, consequently, might play a role in carcinogenesis. The presence of hlyA+ clb+ bacteria in healthy individuals is a potential risk marker of colorectal cancer. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|