Rapid detection of plum pox virus by reverse transcription recombinase polymerase amplification

Autor:	In-Sook Cho, Rae-Dong Jeong, Hwi-Won Jeong, Ho-Jong Ju, Hyo-Jeong Lee
Rok vydání:	2021
Předmět:	0106 biological sciences biology Recombinase Polymerase Amplification Plant Science Horticulture 01 natural sciences Rapid detection Virology Reverse transcriptase 010602 entomology biology.protein Pox virus Viral disease Agronomy and Crop Science Polymerase 010606 plant biology & botany
Zdroj:	Journal of Plant Diseases and Protection. 128:881-885
ISSN:	1861-3837 1861-3829
DOI:	10.1007/s41348-021-00452-z
Popis:	Plum pox virus (PPV) is one of the most destructive viral pathogens infecting stone fruit trees worldwide. As PPV causes a viral disease that requires plants to be quarantined, the development of a reliable method of PPV detection is essential for preventing the spread of the disease. In this study, an isothermal reverse transcription-recombinase polymerase amplification (RT-RPA) assay was developed for the detection of PPV in peaches. The major advantage of this RT-RPA assay is that it can be performed at 42 °C and can be completed in 10 min and has specificity for viruses that infect peaches. In addition, this assay was successfully performed using field-collected samples. This RT-RPA assay is a promising method with high efficiency for rapid PPV detection as part of quarantine inspection and certification program.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::763460b9a40a24ee297567c8c6a6eb5a https://doi.org/10.1007/s41348-021-00452-z Zobrazit plný text záznamu Full text from SpringerLink