Regulation of cellular adhesion and fibronectin synthesis by 1 alpha,25-dihydroxyvitamin D3
| Autor: | R T Franceschi, P R Romano, T C Peter, C J Linson |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
medicine.medical_specialty
biology Cellular differentiation medicine.medical_treatment Cell Cell Biology Cell morphology Biochemistry Fibronectin Steroid hormone Endocrinology medicine.anatomical_structure Cell–cell interaction Cell culture Internal medicine medicine biology.protein Cell adhesion Molecular Biology |
| Zdroj: | Journal of Biological Chemistry. 262:4165-4171 |
| ISSN: | 0021-9258 |
| Popis: | We recently reported that the steroid hormone, 1 alpha,25-dihydroxyvitamin D3 (1,25-(OH)2D3) can inhibit growth, alter morphology, and increase cell associated and medium concentrations of fibronectin (FN) in MG-63 human osteosarcoma cells (Franceschi, R. T., James, W., and Zerlauth, G. (1985) J. Cell. Physiol. 123, 401-409). In the present study, we have tested the hypothesis that 1,25-(OH)2D3 increases cellular adhesion by stimulating FN synthesis. Hormone treatment altered cell morphology and increased cell/substratum adhesion in MG-63 cells, effects which could be mimicked by exogenously added FN. 1,25-(OH)2D3-dependent increases in FN production were due to a rapid (within 12 h) increase in FN synthesis. Maximal (2 to 5-fold) stimulation was observed after 48 h. Hormone treatment did not alter apparent FN stability or distribution during this time. The FN response was specific to 1,25-(OH)2D3 when compared with other vitamin D metabolites. In contrast, triamcinolone acetonide, another known inducer of FN synthesis in certain cells, was only slightly stimulatory up to a concentration of 1 microM. FN mRNA, as measured by Northern blot hybridization, increased within 6 h of 1,25-(OH)2D3 addition with maximal (5-fold) induction seen at 24 h. 1,25-(OH)2D3 also stimulated FN synthesis in several other transformed cell lines (TE-85 human osteosarcomas, SW-480 human colon carcinomas, and HL-60 myeloid leukemia cells). These results may be related to known actions of 1,25-(OH)2D3 on cell differentiation and tumor metastasis. |
| Databáze: | OpenAIRE |
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