Encapsulation of embryonic axes ofCamellia sinensis(L.) O. Kuntze (tea) and subsequentin vitrogermination
| Autor: | M. T. K. Gunasekare, K. Hirimburegama, Thayamini H. Seran |
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| Rok vydání: | 2005 |
| Předmět: | |
| Zdroj: | The Journal of Horticultural Science and Biotechnology. 80:154-158 |
| ISSN: | 2380-4084 1462-0316 |
| DOI: | 10.1080/14620316.2005.11511908 |
| Popis: | SummaryZygotic embryonic axes of Camellia sinensis were coated with 3% and 4% sodium alginate matrices to select the best alginate concentration for the production of synthetic seeds. Germinability and plantlet development of encapsulated and non-encapsulated embryonic axes were evaluated under in vitro conditions. The results revealed that 3% (w/v) sodium alginate with 100 mM calcium chloride dihydrate provided the most suitable matrix of those tested, for encapsulation of embryonic axes. Naked and coated axes, cultured on Murashige and Skoog basal medium alone (MS1) or with 3 mg l–1 benzyl amino purine (BAP) and 0.5 mg l–1 indole butyric acid (IBA), (MS2), gave high rates of survival (100%) and germination (93–100%). However, they responded differently during germination and plant conversion. Somatic embryogenesis was observed in the hypocotyl region of coated and uncoated axes cultured on MS1 medium. Efficient plant recovery from non-encapsulated (73.3%) and encapsulated (42.2%) axes was achieved on MS... |
| Databáze: | OpenAIRE |
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