Repair of Osteochondral Defects in a Rabbit Model Using a Porous Hydroxyapatite Collagen Composite Impregnated With Bone Morphogenetic Protein-2

Autor:	Tomoji Takayama, Hiroaki Yasuda, Atsushi Okawa, Masahiro Hirano, Yuko Kozaka, Xuetao Wei, Shinichi Sotome, Tomokazu Masaoka, Takashi Taniyama, Toshitaka Yoshii, Tsuyoshi Yamada
Rok vydání:	2015
Předmět:	Chemistry Regeneration (biology) Composite number Biomedical Engineering Medicine (miscellaneous) Bioengineering General Medicine Anatomy Bone morphogenetic protein Bone morphogenetic protein 2 Biomaterials Transplantation stomatognathic system JAPANESE WHITE Rabbit model Porosity Biomedical engineering
Zdroj:	Artificial Organs. 39:529-535
ISSN:	0160-564X
DOI:	10.1111/aor.12409
Popis:	Articular cartilage has a limited capacity for spontaneous repair, and an effective method to repair damaged articular cartilage has not yet been established. The purpose of this study was to evaluate the effect of transplantation of porous hydroxyapatite collagen (HAp/Col) impregnated with bone morphogenetic protein-2 (BMP-2). To evaluate the characteristics of porous HAp/Col as a drug delivery carrier of recombinant human BMP-2 (rhBMP-2), the rhBMP-2 adsorption capacity and release kinetics of porous HAp/Col were analyzed. Porous HAp/Col impregnated with different amounts of rhBMP-2 (0, 5, and 25 μg) was implanted into osteochondral defects generated in the patellar groove of Japanese white rabbits to evaluate the effect on osteochondral defect regeneration. At 3, 6, 12, and 24 weeks after operation, samples were harvested and subjected to micro-computed tomography analysis and histological evaluation of articular cartilage and subchondral bone repair. The adsorption capacity was 329.4 μg of rhBMP-2 per cm(3) of porous HAp/Col. Although 36% of rhBMP-2 was released within 24 h, more than 50% of the rhBMP-2 was retained in the porous HAp/Col through the course of the experiment. Defects treated with 5 μg of rhBMP-2 showed the most extensive subchondral bone repair and the highest histological regeneration score, and differences against the untreated defect group were significant. The histological regeneration score of defects treated with 25 μg of rhBMP-2 increased up to 6 weeks after implantation, but then decreased. Porous HAp/Col, therefore, is an appropriate carrier for rhBMP-2. Implantation of porous HAp/Col impregnated with rhBMP-2 is effective for rigid subchondral bone repair, which is important for the repair of the smooth articular surface.
Databáze:	OpenAIRE
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