Ichthyophonus sp. Infection in Opaleye (Girella nigricans)
Autor: | Ashley Mackenzie, Jacob L. Gregg, Maureen K. Purcell, Paul K. Hershberger, Alexandria Mena, Elise E B LaDouceur, Judy St. Leger, William N. Batts |
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Rok vydání: | 2020 |
Předmět: |
0303 health sciences
Budding Girella nigricans General Veterinary biology 040301 veterinary sciences Adipose tissue Spleen Ichthyophonus 04 agricultural and veterinary sciences biology.organism_classification Microbiology law.invention 0403 veterinary science 03 medical and health sciences medicine.anatomical_structure law parasitic diseases medicine Coelom Polymerase chain reaction 030304 developmental biology Explant culture |
Zdroj: | Veterinary Pathology. 57:316-320 |
ISSN: | 1544-2217 0300-9858 |
Popis: | Over a 3-year-period, 17 wild-caught opaleye ( Girella nigricans) housed in a public display aquarium were found dead without premonitory signs. Grossly, 4 animals had pinpoint brown or black foci on coelomic adipose tissue. Histologically, liver, spleen, heart, and posterior kidney had mesomycetozoan granulomas in all cases; other organs were less commonly infected. Four opaleye had goiter; additional substantial lesions were not identified. Granulomas surrounded melanized debris, leukocytes, and mesomycetozoa represented by folded membranes (collapsed schizont walls), intact schizonts (50- to >200 µm in diameter with a multilaminate membrane), plasmodia (budding from schizonts or free in tissue), or rarely germinal tubes (budding from schizonts). Ichthyophonus was grown from fresh tissues in tissue explant broth cultures of the heart, liver, and/or spleen. Polymerase chain reaction using 18S ribosomal DNA primers amplified a 1730-bp region, and the DNA sequence was most similar to Ichthyophonus hoferi, which is often associated with freshwater aquaculture fish. |
Databáze: | OpenAIRE |
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