The Diagnostic Value of Metagenomic Next-Generation Sequencing in Angiostrongylus cantonensis Encephalitis/Meningitis
| Autor: | Hongli Wang, Yongling Song, Qiuyan Peng, Bin Ai, Peiqing Li, Xiaowei Fan, Qiang Wang |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
medicine.medical_specialty
biology business.industry Encephalitis meningitis medicine.disease biology.organism_classification Gastroenterology DNA sequencing Angiostrongylus cantonensis Cerebrospinal fluid Metagenomics Internal medicine medicine biology.protein Antibody business Meningitis Encephalitis |
| Zdroj: | Journal of Behavioral and Brain Science. 11:216-226 |
| ISSN: | 2160-5874 2160-5866 |
| Popis: | Objective: Angiostrongylus cantonensis (A. cantonensis) is an important parasite, which causes neurological infection in children, and is generally difficult to diagnose. We analyzed the significance of metagenomic next-generation sequencing in diagnosing A. cantonensis encephalitis/meningitis in children to provide a reference for clinical diagnosis. Methods: The case collection of A. cantonensis encephalitis/meningitis was from July 2018 to August 2020. The patients’ clinical characteristics and pathogen were described, and diagnostic sensitivity methods for A. cantonensis encephalitis/meningitis were compared and analyzed, including parasite antibody detection and metagenomic next-generation sequencing (mNGS) detection in different samples. Results: Eleven cases were diagnosed with A. cantonensis encephalitis/meningitis, including six males (54.5%) and five females (45.5%), and the age ranged from 1 to 13 years with a median of 21 months (IQR: 15.6, 96). All patients were undiagnosed upon admission, of which ten cases had neurological symptoms or signs, six cases (54.5%) had a history of definite or suspicious exposure to parasites, and eight cases (72.7%) had abnormal changes in cranial MRI. The results of A. cantonensis antibodies in the peripheral blood and cerebrospinal fluid (CSF) were compared with CSF’s metagenomic next-generation sequencing (mNGS). The antibody-positive rate of peripheral blood was 54.5%, CSF antibody-positive rate was 27.2%, and mNGS-positive rate of CSF was 81.8%, and the positive diagnostic rate of the latter was significantly higher than the former two (p = 0.035 p = 0.030 Conclusion: Metagenomic next-generation sequencing has a higher positive rate for diagnosing infection and plays an important role in clarifying diagnosis and reducing misdiagnosis. |
| Databáze: | OpenAIRE |
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