Constructing stable cellular assemblies in the presence of biopolymer: Application of laser tweezing in a crowding medium
| Autor: | Koichiro Sadakane, Kenichi Yoshikawa, Takehiro Yamazaki, Takahiro Kenmotsu |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
chemistry.chemical_classification Scaffold Materials science Nanotechnology Polymer engineering.material Laser law.invention 03 medical and health sciences 030104 developmental biology Solid substrate Assembly systems Tissue engineering chemistry Optical tweezers law engineering Biopolymer |
| Zdroj: | MHS |
| DOI: | 10.1109/mhs.2018.8886996 |
| Popis: | We propose a novel methodology for constructing a stable three-dimensional cellular assembly by laser tweezer under depletion interaction in a natural polymer solution, without the usage any artificial scaffold such as gel or solid substrate. Successful construction of 3D cellular assembly is attained by forming stable cell-cell contacts within a few minutes. Our newly established method is expected to be useful for next-generation 3D single-cell assembly systems in the field of regenerative medicine and tissue engineering. The present study shows a concrete further extension of our recent reports on the use of soluble polymers for the construction of 3D single-cell assemblies. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|