Determination of Tilmicosin in Swine Feeds by Liquid Chromatography

Autor:	Robin S. Readnour, Sharon S Dixon, Susan L. Helton-Groce
Rok vydání:	1997
Předmět:	Pharmacology Matrix (chemical analysis) chemistry.chemical_compound Chromatography chemistry Environmental Chemistry Tilmicosin Agronomy and Crop Science Food Science Analytical Chemistry
Zdroj:	Journal of AOAC INTERNATIONAL. 80:1161-1170
ISSN:	1944-7922 1060-3271
DOI:	10.1093/jaoac/80.6.1161
Popis:	This method determines tilmicosin in feeds over a concentration range of 100 to 600 mg/kg. Tilmicosin is extracted from swine feeds by adding 200 mL of a swine feed extractant (20 + 80, acetonitrile–Millipore water, pH 2.5, with 25 mM dibutylammonium phosphate) to 20 g feed and placing on a shaker table for 1 h. This extractant is filtered and analyzed by liquid chromatography (LC). A gradient LC method is used to separate ilmicosin from the feed matrix in 30 min of run time. The recovery of tilmicosin from fortified feeds ranged from 96.7 to 112%, with the coefficients of variation (CVs) ranging from 1.4 to 3.9%. The determination of tilmicosin in medicated feeds resulted in an average recovery of 92.7% of labeled claim for pelleted feeds at 200 mg/kg and 99.1% of labeled claim for mash feeds at 400 mg/kg. Determination of tilmicosin in medicated feeds resulted in CVs ranging from 2.6 to 3.8%. The method has shown no interference with 18 other drugs.
Databáze:	OpenAIRE
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