Enumeration and functional investigation of endothelial progenitor cells in neovascularization of diabetic foot ulcer rats with a Chinese 2-herb formula
| Autor: | Clara Bik-San Lau, Hin Fai Kwok, Kwok-Pui Fung, Jacqueline Chor Wing Tam, Ping-Chung Leung, Ming Ho To, Wai-Yee Chan, Wing-Sum Siu, Chun-Hay Ko, Ching Po Lau, Kit Man Lau |
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| Rok vydání: | 2014 |
| Předmět: |
Tube formation
medicine.medical_specialty Stromal cell Cluster of differentiation business.industry Endocrinology Diabetes and Metabolism CD34 medicine.disease Surgery Andrology Neovascularization Diabetic foot ulcer embryonic structures cardiovascular system medicine CD146 Progenitor cell medicine.symptom business circulatory and respiratory physiology |
| Zdroj: | Journal of Diabetes. 7:718-728 |
| ISSN: | 1753-0393 |
| Popis: | Backbround We investigated the effect of a Chinese 2-herb formula (NF3) on the enumeration and angiogenic differentiation of endothelial progenitor cells (EPCs) in diabetic foot ulcer rats. Methods EPCs and stromal cell-derived factor-1α (SDF-1α) were quantified by flow cytometry and ELISA, respectively. In vitro angiogenesis assays included proliferation, adhesion, migration and tube formation. Results Our result demonstrated that NF3 (0.98 g/kg) could significantly enhance the circulating CD34+/VEGFR2+/CD45− EPCs levels in diabetic foot ulcer rats by 60% (P |
| Databáze: | OpenAIRE |
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