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The experiment was planned to assess the supplemental feeding of 40 g/day (35% w/w choline) rumen protected choline (T2) in total mixed ration from − 21 to 120 days of calving to dairy cows on lactation performance of 294 days compared to control TMR (T1). Total sixteen cows were allotted in two groups having six multiparous and two primiparous cows. The multiparous cows allocated based on previous standard lactation yield and lactation number. The primiparous cows allocated based on the first standard lactation yield of dam. An intake, milk production was calculated at 21 biweekly period of lactation. The milk composition assessed four times during lactation. The daily intake of dry matter, total digestible nutrients was significantly lower by 2.44 kg and 1.46 kg, respectively in T2 group of cows compared to control. The cows of T2 group produced significantly (P |