Chimerism Testing by Quantitative PCR Using Indel Markers

Autor:	David Senitzer, Laima Gaidulis, Ketevan Gendzekhadze
Rok vydání:	2013
Předmět:	surgical procedures operative Real-time polymerase chain reaction Genetic marker medicine.medical_treatment Immunology Donor chimerism medicine Early detection Hematopoietic stem cell transplantation Biology Indel Highly sensitive
Zdroj:	Methods in Molecular Biology ISBN: 9781627034920
DOI:	10.1007/978-1-62703-493-7_11
Popis:	Engraftment monitoring is critical for patients after Hematopoietic Stem Cell Transplantation (HSCT). Complete donor chimerism is the goal; therefore, early detection of rejection and relapse is crucial for guiding the patient post HSCT treatment. Quantitative PCR for chimerism testing has been reported to be highly sensitive. In this chapter we discuss the quantitative PCR (qPCR) method using 34 Indel (Insertion and Deletion) genetic markers spread over 20 different chromosomes.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::75b3b8d6e88f6f3b20ede9e493e59b82 https://doi.org/10.1007/978-1-62703-493-7_11 Zobrazit plný text záznamu