CLINICAL SIGNIFICANCE OF METHYLATION OF A GROUP OF MIRNA GENES IN OVARIAN CANCER PATIENTS
| Autor: | D. N. Kushlinsky, Vitaly I. Loginov, N. E. Kushlinskii, E. A. Filippova, Eleonora A. Braga, A. M. Burdennyy, I. V. Pronina, D O Utkin |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Biochemistry (medical) MicroRNA Gene Cancer General Medicine Methylation Biology medicine.disease 03 medical and health sciences Medical Laboratory Technology 030104 developmental biology 0302 clinical medicine CpG site 030220 oncology & carcinogenesis microRNA Ascites medicine Cancer research Clinical significance medicine.symptom Ovarian cancer |
| Zdroj: | Russian Clinical Laboratory Diagnostics. 65:321-327 |
| ISSN: | 0869-2084 |
| DOI: | 10.18821/0869-2084-2020-65-5-321-327 |
| Popis: | It was found that the proportion of microRNA genes inactivated by methylation of regulatory CpG islands is several times higher than the genes encoding proteins, which increases their attractiveness as promising markers of cancer. The aim of this work is to evaluate the clinical significance of methylation of 13 tumor-associated microRNA genes (MIR-124a-2, MIR-124a-3, MIR-125-B1, MIR-127, MIR-129-2, MIR-132, MIR-137, MIR-203a, MIR-34b/c, MIR-375, MIR-9-1, MIR-9-3, MIR-339) in 26 patients with ovarian cancer. Methylation level was evaluated by the method of methylation-specific PCR in real time. The data obtained in primary tumors (26), histologically unchanged ovarian tissues (15) and peritoneal metastases (19) were compared using a number of statistical programs. For all 13 genes, an increase in the level of methylation was revealed during the transition from unchanged tissue to primary tumors and further from primary tumors to peritoneal metastases; moreover, in the genes MIR-203a, MIR-375 and MIR-339, the level of methylation in metastases increased most significantly (in 2 and more times). A correlation was observed for the first time, showing a consistency between the increase in methylation level in some miRNA pairs, for example, MIR-129-2/MIR-132 (rs> 0,7; p |
| Databáze: | OpenAIRE |
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