Determination of Mycotoxins in Cereal-Based Porridge Destined for Infant Consumption by Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry
| Autor: | Maria Heloísa Paulino de Moraes, Yuri Pereira Souza, Armi Wanderley da Nóbrega, Rosana Pereira dos Santos, André Victor Sartori |
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| Rok vydání: | 2017 |
| Předmět: |
Ochratoxin A
Aflatoxin Chromatography 010401 analytical chemistry 04 agricultural and veterinary sciences Mass spectrometry 040401 food science 01 natural sciences Applied Microbiology and Biotechnology 0104 chemical sciences Analytical Chemistry chemistry.chemical_compound 0404 agricultural biotechnology Vomitoxin chemistry Liquid chromatography–mass spectrometry Food science Safety Risk Reliability and Quality Mycotoxin Safety Research Zearalenone Food Science Sterigmatocystin |
| Zdroj: | Food Analytical Methods. 10:4049-4061 |
| ISSN: | 1936-976X 1936-9751 |
| DOI: | 10.1007/s12161-017-0965-4 |
| Popis: | An analytical method applying ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) for the determination of aflatoxins M2, M1, G2, G1, B2, B1, deoxynivalenol, ochratoxin A, fumonisins B1 and B2, hydrolyzed fumonisins B1 and B2, zearalenone and sterigmatocystin in cereal-based porridge destined for infant consumption was developed and validated. The mycotoxins were extracted using an adequate solvent ratio (n-hexane/3% formic acid solution/acetonitrile) under rapid shaking and sonication, without any further cleanup steps. Recoveries ranged from 63.5 to 113.2% and were considered satisfactory, with relative standard deviations lower than 20%. The limits of quantification ranged from 0.14 to 6.73 μg kg−1. The validated method was then applied to the determination of mycotoxins in 84 samples of cereal-based porridge destined for infant consumption obtained in the metropolitan region of Rio de Janeiro, RJ. Aflatoxins B1 and G1, fumonisins B1 and B2, hydrolyzed fumonisins B1 and B2, deoxynivalenol, and zearalenone were found, respectively, in 7.1, 2.4, 47.6, 3.6, 65.5, and 60.7% of the analyzed samples. The maximum permissible limits set by the Brazilian legislation were exceeded for at least one mycotoxin in 21 (25%) of the analyzed samples. |
| Databáze: | OpenAIRE |
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