Targeting SARS-CoV-2 RNA-dependent RNA polymerase: An in silico drug repurposing for COVID-19
| Autor: | Chetan Hasmukh Mehta, Akhil Suresh, Yogendra Nayak, Usha Y. Nayak, Krishnaprasad Baby, Swastika Maity |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Antagonists & inhibitors General Immunology and Microbiology Drug discovery business.industry viruses In silico RNA-dependent RNA polymerase General Medicine Virology General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Drug repositioning 030104 developmental biology 0302 clinical medicine Drug development Docking (molecular) 030220 oncology & carcinogenesis Medicine Rosoxacin General Pharmacology Toxicology and Pharmaceutics business medicine.drug |
| Zdroj: | F1000Research. 9:1166 |
| ISSN: | 2046-1402 |
| DOI: | 10.12688/f1000research.26359.1 |
| Popis: | Background: The coronavirus disease 2019 (COVID-19) pandemic, caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), took more lives than combined epidemics of SARS, MERS, H1N1, and Ebola. Currently, the prevention and control of spread are the goals in COVID-19 management as there are no specific drugs to cure or vaccines available for prevention. Hence, the drug repurposing was explored by many research groups, and many target proteins have been examined. The major protease (Mpro), and RNA-dependent RNA polymerase (RdRp) are two target proteins in SARS-CoV-2 that have been validated and extensively studied for drug development in COVID-19. The RdRp shares a high degree of homology between those of two previously known coronaviruses, SARS-CoV and MERS-CoV. Methods: In this study, the FDA approved library of drugs were docked against the active site of RdRp using Schrodinger's computer-aided drug discovery tools for in silico drug-repurposing. Results: We have shortlisted 14 drugs from the Standard Precision docking and interaction-wise study of drug-binding with the active site on the enzyme. These drugs are antibiotics, NSAIDs, hypolipidemic, coagulant, thrombolytic, and anti-allergics. In molecular dynamics simulations, pitavastatin, ridogrel and rosoxacin displayed superior binding with the active site through ARG555 and divalent magnesium. Conclusion: Pitavastatin, ridogrel and rosoxacin can be further optimized in preclinical and clinical studies to determine their possible role in COVID-19 treatment. |
| Databáze: | OpenAIRE |
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