Quantification of Porcine Complement Activation Fragment C3a by a Neoepitope-Based Enzyme-Linked Immunosorbent Assay
| Autor: | Martin Oppermann, Kristin Pettersen, Hilde Fure, Espen Waage Skjeflo, Tom Eirik Mollnes, Dorte Christiansen, Per H. Nilsson |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
chemistry.chemical_classification Analyte biology chemical and pharmacologic phenomena 030208 emergency & critical care medicine Molecular biology 3. Good health Complement system 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Enzyme chemistry biology.protein Anaphylatoxin Antibody |
| Zdroj: | The Complement System ISBN: 9781071610152 |
| DOI: | 10.1007/978-1-0716-1016-9_5 |
| Popis: | Enzyme-linked immunosorbent assay (ELISA) enables fast and simple quantification of analytes in the pico- to nanogram range in complex samples. Here, we describe an ELISA for the detection of porcine C3a as a marker for complement activation. Antibody specificity is critical for a robust assay. This assay is based on a pair of antibodies specific for the porcine C3a molecule and thus does not react with native C3. |
| Databáze: | OpenAIRE |
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