Development of a paper-type tyrosinase biosensor for detection of phenolic compounds

Autor: V. Cengiz Ozalp, M. Tufan Oz, Yakup M. Arıca, Remziye Yılmaz, Füsun Eyidoğan, Hüseyin Avni Öktem, Ozge Senyurt
Rok vydání: 2014
Předmět:
Zdroj: Biotechnology and Applied Biochemistry. 62:132-136
ISSN: 0885-4513
Popis: A low-cost, portable, and disposable paper-type tyrosinase biosensor was developed for determination of phenolic compounds, using a paper-strip absorption method. Tyrosinase and a chromophore (3-methyl-2-benzothiazolinone hydrazone) were immobilized on paper strips to manufacture the biosensor, which was tested on a nontoxic substrate (l-dopamine). The biosensor was responsive to phenolic compounds such as 4-chlorophenol, catechol, m-cresol, and p-cresol. The sensor showed stability for 70 days. The developed biosensor can be used for remote on-site qualitative monitoring of phenolic compounds in wastewater samples.
Databáze: OpenAIRE