Optimization of medium components to improve the antimicrobial activity of a new Saharan actinobacterial strain Saccharothrix tamanrassetensis DSM 45947
| Autor: | Hadjira Boudjella, Keramane Leila Bakour, Nouredinne Bouras, Florence Mathieu |
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| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | Research Journal of Biotechnology. 16:35-47 |
| ISSN: | 2278-4535 0973-6263 |
| DOI: | 10.25303/167rjbt3521 |
| Popis: | Saccharothrix tamanrassetensis DSM 45947 previously isolated from a Saharan soil sample and characterized as a new species, was found to be a potential candidate in the search for novel antibiotics. The strain was found to exhibit a strong antimicrobial activity against a wide range of bacteria and fungi. In this study, statistical methods were applied to optimize the fermentation medium for enhanced antimicrobial activity production. A basal medium supplemented with sucrose and corn steep liquor was used as the original medium for optimization experiments. Plackett-Burman design showed that sucrose, corn steep liquor and KH2PO4, were recognised to have significant effect on antimicrobial activity production. Response surface methodology with Box-Behnken design determined the optimal concentrations. Optimal values for maximum antibiotic production were as follows: 7 g L-1 of sucrose, 52 g L-1 of corn steep liquor and 0.9 g L-1 of KH2PO4. Under these conditions, antimicrobial activities of DSM 45947 showed a global increase by 32% against the bacterium methicillinresistant Staphylococcus aureus and 36% against the fungus Umbelopsis ramanniana. This study provided an economical and efficient method to cultivate Saccharothrix tamanrassetensis DSM 45947 for enhanced antimicrobial activity production. |
| Databáze: | OpenAIRE |
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