Equipment contact surfaces as sources of Staphylococcus carrying enterotoxin-encoding genes in goat milk dairy plants
| Autor: | Mauro M.S. Saraiva, Núbia Michelle Vieira da Silva, Patrícia Emília Naves Givisiez, Celso José Bruno de Oliveira, Rafael Felipe da Costa Vieira, Candice M C G de Leon, Francisca G.C. Sousa |
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| Rok vydání: | 2020 |
| Předmět: |
0402 animal and dairy science
Dairy milk 04 agricultural and veterinary sciences Enterotoxin Biology Contamination medicine.disease_cause 040401 food science 040201 dairy & animal science Applied Microbiology and Biotechnology law.invention 0404 agricultural biotechnology Contact surfaces law Genotype medicine Food science Gene Staphylococcus Polymerase chain reaction Food Science |
| Zdroj: | International Dairy Journal. 111:104827 |
| ISSN: | 0958-6946 |
| DOI: | 10.1016/j.idairyj.2020.104827 |
| Popis: | Potential contamination sources and the enterotoxigenic profiling of Staphylococcus isolates were investigated in four goat dairy plants. Samples (n = 160) were collected from different processing points in five samplings. Staphylococcus spp. isolates were confirmed by polymerase chain reaction and genotyped using repetitive extragenic palindromic PCR (Rep-PCR). Classical (sea, seb, sec, sed) and novel (seg, seh, sei) staphylococcal enterotoxin-encoding genes were detected by PCR. seb, sec, see and sei were identified in isolates originated from raw and pasteurised milk, pre- and post-pasteurisation tanks and wall surfaces. SE-harbouring isolates were mostly identified as non-aureus staphylococci. Genotypic profiles and contamination levels indicate that the packaging machine is a critical point for staphylococcal contamination. Equipment surfaces are important contamination sources to pasteurised milk considering the indistinguishable genotypic patterns of isolates from pre- and post-pasteurisation points. Cleaning and disinfection of milk contact surfaces should be revisited to warrant quality and safety of goat milk. |
| Databáze: | OpenAIRE |
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