Comparative Cytotoxicity Study of Cisplatin and its Metabolites by using Brine Shrimp Lethality Bioassay

Autor:	Nishat Parvin, Anwar Ul Islam, Akm Shahidur Rahman, Mir Misbahuddin, Moniruzzaman Khan
Rok vydání:	2017
Předmět:	Cisplatin Kidney business.industry Cancer Pharmacology medicine.disease Nephrotoxicity medicine.anatomical_structure Toxicity Immunology medicine Bioassay Ovarian cancer business Cytotoxicity medicine.drug
Zdroj:	KYAMC Journal. 8:15-20
ISSN:	2308-2860 2308-2720
Popis:	Background: Cisplatin an old chemotherapeutic agent used to treat various types of cancers, including sarcomas, some carcinomas e.g. small cell lung cancer and ovarian cancer, lymphomas and germ cell tumors etc. Most important toxicity of cisplatin is nephrotoxicity produced by cisplatin itself and its few metabolites, which are some time fatal. Objectives: Present study was to determined the comparative study and the level of toxicities of cisplatin and their metabolires by using Brine Shrimp Lethality Bioassay. Methods: In this study, cisplatin and its four possible metabolites (CM2 to CM5) were isolated from the 24 hours collected urines of 5 cisplatin treated cancer patients using conventional chromatographic techniques as well as HPLC, were placed to comparative cytotoxic study using brine shrimp lethality bioassay. Results: Comparative to cisplatin, its metabolites are more toxic, specially CM2 (Meta 2, LC50=1.2?gm/ml) and CM3 (LC50=1.08 ?gm/ml), CM4 (Meta 4, LC50=1.182?gm/ml) which is correlate to the previous study (p
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::7569a03c298a642fa251b06f4462f061 https://doi.org/10.3329/kyamcj.v8i1.33867 Zobrazit plný text záznamu