| Popis: |
The effect of ionizing radiation on the blood parameters of monkeys (rhesus monkeys, male species) was studied. Animals were exposed to irradiation with 1/10 of the total dose (50 cSv) for 10 days with an interval of 2 days (Group-1) or 1/2 of the total dose (50 cSv) for 2 days (Group-2). It was found that decrease in the hematocrit was more pronounced on the 36th day during the first irradiation, and on the 64th day after the second one. As a rule, a decrease in hemoglobin content was observed after the first and second irradiations, which could indicate an alteration in the hematopoietic system function. Molecular alterations in blood plasma carotenoids and erythrocyte hemoglobin heme were studied using the Raman spectroscopy. It was proven that after irradiation the microenvironment of carotenoids in plasma lipoprotein complexes in Group-2 became less viscous, than in Group-1. This indicated that on the 36th day processes were triggered in blood plasma of the Group-2 animals that changed the nature of protein-lipid interactions in plasma lipoprotein complexes, where carotenoids were localized. On certain days after irradiation, reliable alterations were registered in the erythrocytes hemoglobin properties in Group-1 and Group-2 compared to each other. It is likely that the second irradiation had a different effect on the erythrocyte hemoglobin: Group-1 irradiation had a more pronounced effect on the erythrocyte hemoglobin than in Group-2. Difference in the erythrocyte hemoglobin affinity to oxygen observed in Group-1 and Group-2 after the first irradiation was not correlating with alterations in the hematocrit or erythrocyte hemoglobin content in blood, but was associated with other processes that affected the erythrocyte hemoglobin conformation. According to the authors of the work, results of studying the carotenoid molecules content or conformation in the blood plasma lipoprotein complexes would allow developing and introducing not only a methodology for diagnosing the state of an organism as a whole, but also the therapy using the natural antioxidants |
