| Autor: |
D.A. Doornbos, Jan H. De Boer, Jan Bosman, Rokus A. de Zeeuw, Dirk T. Witte |
| Rok vydání: |
1991 |
| Předmět: |
|
| Zdroj: |
Journal of Chromatography A. 558:333-342 |
| ISSN: |
0021-9673 |
| DOI: |
10.1016/0021-9673(91)80001-w |
| Popis: |
The applicability of a Chiralcel OD column for the separation of the enantiomers of the racemic dopamine agonist 2- (N-propyl-N-2-thienylethylamino)-5-hydroxytetralin (N-0437) was studied. The resolution between the enantiomers was described by means of the response surface methodology and optimum chromatographic conditions were found using Smilde's multi-criteria decision-making technique. The variables studied were eluent composition, flow-rate and temperature. The effects of these variables on the retention time of the last-eluting enantiomer and the resolution between the enantiomers were examined. The optimum result was considered to be the highest resolution possible within a short retention time for the last-eluting enantiomer. It appeared that a decrease in temperature gave higher resolutions. With hexane-ethanol (95:5, v/v) as eluent at 10°C and a flow-rate of 0.5 ml/min, a resolution of 2.5 and a retention time of the last-eluting enantiomer of 23 min were obtained. Under these conditions calibration graphs for the (+)- and (−)-enantiomers were prepared using racemic N-0437. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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