| Autor: |
Yoshimichi Nakatsu, Yuta Arakawa, Ryotaro Tanaka, Yoko Yamanishi |
| Rok vydání: |
2017 |
| Předmět: |
|
| Zdroj: |
2017 IEEE 30th International Conference on Micro Electro Mechanical Systems (MEMS). |
| DOI: |
10.1109/memsys.2017.7863438 |
| Popis: |
We have succeeded in injection of plasmid to adherent cells which are suspended in the plasma-bubbles laden circulation flow in a chamber. High-speed plasma-bubbles are generated by glass electrode and the air-liquid interface has a stiction force which draws the gene (plasmid) and stick to the air-liquid interface. The circulating flow increased the chance for cells to contact air-liquid interface of bubbles which enclosed plasma or reactive gas. Finally, the high reactive interface enables gene transfer to cells efficiently. This technology of two-dimensional microfluidic chip contributes an option to the high-throughput gene transfer. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
