Growth Characteristics of Rickettsia Species LON Strains Closely Related to Rickettsia japonica Isolated from Haemaphysalis longicornis Ticks in Mouse Derived L929 and Human-Derived THP-1 Host Cell Lines
| Autor: | Ai Takano, Hiromi Fujita, Hongru Su, Naoya Takamoto, Fuyuki Abe, Norio Ohashi, Saori Oishi, Shuji Ando, Hitoshi Tai |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Rickettsia japonica biology Host (biology) 030106 microbiology General Medicine biology.organism_classification medicine.disease Japonica Microbiology 03 medical and health sciences Open reading frame chemistry.chemical_compound 0302 clinical medicine Infectious Diseases chemistry medicine bacteria Japanese spotted fever THP1 cell line 030212 general & internal medicine Haemaphysalis longicornis DNA |
| Zdroj: | Japanese Journal of Infectious Diseases. 74:102-109 |
| ISSN: | 1884-2836 1344-6304 |
| DOI: | 10.7883/yoken.jjid.2020.444 |
| Popis: | Non-pathogenic Rickettsia species LON strains closely related to an agent of Japanese spotted fever (JSF), R. japonica, were isolated in Japan from Haemaphysalis longicornis ticks in 2001. However, the biological properties of LONs in mammalian host cells are poorly understood. In this study, microscopic analysis showed that LONs in a mouse-derived L929 host cell line were rod shaped with sizes of 0.3-0.5 × 0.5-2.0 μm. Molecular analysis revealed the existence of a LON-specific disrupted open reading frame in R. japonica-related group-specific DNA regions. Growth kinetics of LON-2 and LON-13 strains analyzed by a quantitative real-time PCR showed 100-fold or more increment of LONs cultured in L929 host cells at 30°C and slightly less increment at 33°C, and 25-fold increment in human-derived THP-1 host cells at 35°C on day 7 (168 h) post infection. The generation times of the two LON strains cultured in L929 and THP-1 were estimated to be 9.4-12.9 h and 9.6-10.9 h, respectively. To our knowledge, this is the first report on the biological characteristics of Rickettsia sp. LON strains in mammalian cells, which may provide significant information for the experimental approaches for other rickettsiae. |
| Databáze: | OpenAIRE |
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