Development of reverse-phase high-performance liquid chromatography method for simultaneous determination of sodium benzoate and potassium sorbate in beverages
| Autor: | Lenche Velkoska-Markovska, Biljana Petanovska-Ilievska, Mirjana Jankulovska |
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| Rok vydání: | 2017 |
| Předmět: |
Detection limit
Preservative Chromatography Potassium sorbate 010401 analytical chemistry 04 agricultural and veterinary sciences General Chemistry 040401 food science 01 natural sciences High-performance liquid chromatography 0104 chemical sciences chemistry.chemical_compound 0404 agricultural biotechnology chemistry Surface-area-to-volume ratio Phase (matter) Sodium benzoate Potassium benzoate |
| Zdroj: | Acta Chromatographica. 29:345-358 |
| ISSN: | 2083-5736 1233-2356 |
| DOI: | 10.1556/1326.2017.29.3.05 |
| Popis: | Reverse-phase high-performance liquid chromatography (RP-HPLC) method for simultaneous determination of sodium benzoate and potassium sorbate in beverages was developed using high speed column. The simple and rapid reverse-phase method for quantitative determination of both preservatives was established on LiChroCART® Purospher STAR RP-18e (30 mm × 4 mm; 3 μm) column, mobile phase consisted of acetonitrile-phosphate buffer (pH = 3.5) in volume ratio of 8:92 (v/v), flow rate of 1 mL min−1, ultraviolet (UV) detection at 195 nm for sodium benzoate and 260 nm for potassium sorbate, and constant column temperature at 25 °C. Linearity, precision, accuracy, limit of quantification (LOQ), and limit of detection (LOD) were tested for method validation. Linearity range for sodium benzoate was 6.04–200.27 mg L−1 (R2 = 0.999) while, for potassium benzoate (R2 = 0.999), 12.19–406.36 mg L−1. The RSD values ≤1.03% demonstrate excellent intra-day precision. LOD for sodium benzoate and potassium sorbate was 0.004 and 0.00... |
| Databáze: | OpenAIRE |
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