Selection of Bacillus thuringiensis against Pathogenic Nematodes Attacking Pepper Tree
Autor: | Tuan Vo Chau, Ha Do Thu, Minh Vo Van, Trang Le Vu Khanh, My Pham Тhi |
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Rok vydání: | 2020 |
Předmět: |
0106 biological sciences
0303 health sciences Ecology 030302 biochemistry & molecular biology Biology biology.organism_classification 01 natural sciences Applied Microbiology and Biotechnology 03 medical and health sciences Tree (data structure) Horticulture 010608 biotechnology Bacillus thuringiensis Pepper Selection (genetic algorithm) Biotechnology |
Zdroj: | Biotekhnologiya. 36:57-62 |
ISSN: | 2500-2341 0234-2758 |
DOI: | 10.21519/0234-2758-2020-36-3-57-62 |
Popis: | Nine strains belonging to the Bacillus genus have been isolated from soil samples in Tien Phuoc district, Quang Nam province. They were capable of surviving at 42°C, and the Bl, B4, B7 and B9 strains could produce toxic crystals at this temperature. B7 had the most promising characteristics in terms of spore-forming ability. The result of the 16S rRNA gene sequencing showed that B7 belongs to the Bacillus thuringiensis species, which is known to effectively control root-knot Meloidogyne sp. nematodes attacking pepper tree. This study was aimed at evaluating the inhibitory effect of the bacterial isolate under study on root-knot eggs and juveniles. It was shown that the highest inhibitory activity of the cultures of the bacterial strains under study was observed at their concentration of 109cells/mL; in this case, up to 89.67% of nematode eggs and 100% of juveniles J2 were killed after 10 h of treatment. Bacillus thuringiensis, Meloidogyne sp., pepper tree, root-knot, nematodes We are grateful to the Danang University of Science and Education and Duy Tan University for supporting this study. |
Databáze: | OpenAIRE |
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