Selection of Bacillus thuringiensis against Pathogenic Nematodes Attacking Pepper Tree

Autor: Tuan Vo Chau, Ha Do Thu, Minh Vo Van, Trang Le Vu Khanh, My Pham Тhi
Rok vydání: 2020
Předmět:
Zdroj: Biotekhnologiya. 36:57-62
ISSN: 2500-2341
0234-2758
DOI: 10.21519/0234-2758-2020-36-3-57-62
Popis: Nine strains belonging to the Bacillus genus have been isolated from soil samples in Tien Phuoc district, Quang Nam province. They were capable of surviving at 42°C, and the Bl, B4, B7 and B9 strains could produce toxic crystals at this temperature. B7 had the most promising characteristics in terms of spore-forming ability. The result of the 16S rRNA gene sequencing showed that B7 belongs to the Bacillus thuringiensis species, which is known to effectively control root-knot Meloidogyne sp. nematodes attacking pepper tree. This study was aimed at evaluating the inhibitory effect of the bacterial isolate under study on root-knot eggs and juveniles. It was shown that the highest inhibitory activity of the cultures of the bacterial strains under study was observed at their concentration of 109cells/mL; in this case, up to 89.67% of nematode eggs and 100% of juveniles J2 were killed after 10 h of treatment. Bacillus thuringiensis, Meloidogyne sp., pepper tree, root-knot, nematodes We are grateful to the Danang University of Science and Education and Duy Tan University for supporting this study.
Databáze: OpenAIRE