Research on the regulatory mechanisms of self-incompatibility plasticity in Nicotiana alata
| Autor: | Xuemei Wei, Zihui Pan, Jugou Liao, Zhiyun Chen, Xiaojun Qin, Yongzhi Niu, Suo Wenlong, Yunye Zheng, Wenguang Ma, Suiyun Chen |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Senescence Plant senescence Gynoecium Nicotiana alata biology medicine.diagnostic_test food and beverages Plant Science Horticulture medicine.disease_cause biology.organism_classification 01 natural sciences Phenotype Cell biology 03 medical and health sciences 030104 developmental biology Western blot Transcription (biology) Pollen Genetics medicine Agronomy and Crop Science 010606 plant biology & botany |
| Zdroj: | Euphytica. 216 |
| ISSN: | 1573-5060 0014-2336 |
| DOI: | 10.1007/s10681-020-02687-7 |
| Popis: | Nicotiana alata is a model species used to study gametophytic self-incompatibility (SI), controlled by the S-locus (encoding S-RNase and SLF proteins expressed in pistil and pollen, respectively) as well as by modifier genes (such as 120 kDa, HT-b and Trxh). A previous study revealed that SI in N. alata accession S2S2 was plastic, and that SI was leaky with senescence of the pistil and plant. Here, we further explored its regulatory mechanisms. Reverse transcription quantitative PCR (RT-qPCR) and western blot results showed that during the senescence of pistil in S2S2, 120 kDa and HT-b were transcribed and translated at lower levels, while marked residual S-RNase activity was detected. The transcripts of Trxh varied irregularly. Only the transcription and translation of 120 kDa declined with the plant senescence. According to the results, PSF in S2S2 may be associated with down-regulated expression of 120 kDa and HT-b, while S-RNase and Trxh seem to be unrelated to this phenotype. These findings deepen our understanding of the regulatory mechanisms of SI plasticity in N. alata. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink