The potential role of locally induced matrix metalloproteinase 3 in respiratory mucosal immunity (39.17)

Autor: Xueshui Guo, David E. B. Knudsen, Xiuqing Wang
Rok vydání: 2009
Předmět:
Zdroj: The Journal of Immunology. 182:39.17-39.17
ISSN: 1550-6606
0022-1767
Popis: Recent studies suggest that matrix metalloproteinase 3 (MMP3) is involved in intestinal immunity against bacterial infections as well as in immune cell migrations in delayed hypersensitivity. The goal of this study is to assess the potential role of MMP3 in the development of mucosal immunity at respiratory tract using CpG oligodeoxynucleotide (ODN) as a model system. Groups of BALB/c mice were administrated intranasally with either CpG ODN or GpC ODN or PBS. MMP3 was up-regulated at both 1 and 3 days post immunization in bronchoalveolar lavages (BAL) and respiratory mucosal tissues of CpG ODN immunized mice as determined by ELISA, Western blot and immunohistochemistry (IHC) staining. Furthermore, an increase of both CD11c+ antigen presenting cells and CD4+ T cells was observed in lungs of CpG vaccinated mice compared with GpC ODN or PBS controls. To confirm that MMP3 expression is associated with increased immune cell migration in lungs, mice were treated with MMP3-specific inhibitor II 6hr after CpG immunization. Lung tissues were collected 3 days later and subjected to IHC analysis to determine the expression of MMP3 and the number of CD11c antigen presenting cells and CD4+ T cells. Results showed that MMP3 expression was significantly reduced after treatment with inhibitor II, suggesting that MMP3 inhibitor effectively reduced the expression of MMP3. Accordingly, a reduced number of CD11c+ antigen presenting cells and CD4+ T helper cells was observed in inhibitor-treated mice, indicating a possible role of MMP3 in immune cell migration. Ongoing experiments are focused on validating these observations and investigating the mechanistic basis of this MMP3-associated mucosal immunity. It is anticipated that the results may provide new insights on the molecular basis of mucosal immunity at respiratory tract. (Supported by NSF/EPSCoR EPS-0091948)
Databáze: OpenAIRE
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