| Autor: |
L. Cronenberger, Jean-Noël Bidard |
| Rok vydání: |
1979 |
| Předmět: |
|
| Zdroj: |
Journal of Chromatography B: Biomedical Sciences and Applications. 164:139-154 |
| ISSN: |
0378-4347 |
| DOI: |
10.1016/s0378-4347(00)81183-4 |
| Popis: |
Identification of 4-O-methyldopamine in rat tissues by reversed-phase liquid chromatography 4-O-Methyldopamine was identified and assayed in tissues from l -dopa treated rats by reversed-phase high-performance liquid chromatography. The initial steps in the separation of catecholamines were performed by alumine, a weak cation-exchange resin, and thin-layer chromatographic techniques. After L-[3H] dopa administration, the radiochromatogram was superimposed on the fluorochromatogram obtained with authentic marker 4-O-methyldopamine. This metabolite was detected in kidney but not in brain. The 4-O-methyldopamine:3-O-methyldopamine ratio was 0.032 in kidney. The influence of various treatments on this ratio was investigated. A 160% increase was found after l -dopa administration. This effect was potentiated by nialamide pretreatment (550% increase). |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
