Mammalian adipose tissue and muscle are major sources of lipid transfer protein mRNA
| Autor: | Ira J. Goldberg, R Schnitzer-Polokoff, D Compton, E. Quinet, Liliane K. Yacoub, P. Moulin, Xuliang Jiang, Alan R. Tall, Luis B. Agellon |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
medicine.medical_specialty
Lipoprotein lipase Triglyceride biology Cholesterol nutritional and metabolic diseases Adipose tissue Cell Biology Biochemistry carbohydrates (lipids) chemistry.chemical_compound Endocrinology chemistry Internal medicine Cholesterylester transfer protein medicine biology.protein Cholesteryl ester Lipolysis lipids (amino acids peptides and proteins) Molecular Biology Plant lipid transfer proteins |
| Zdroj: | Journal of Biological Chemistry. 266:4631-4639 |
| ISSN: | 0021-9258 |
| DOI: | 10.1016/s0021-9258(20)64369-1 |
| Popis: | The plasma cholesteryl ester transfer protein (CETP) catalyzes the transfer of cholesteryl esters from high density lipoproteins (HDL) to triglyceride-rich lipoproteins and plays a major role in the catabolism of HDL. Lipoprotein lipase (LPL) is the rate-limiting enzyme for hydrolysis of circulating triglyceride and is involved in HDL formation. We show that tissues containing LPL are major sources of CETP mRNA in several mammalian species, including some with low cholesteryl ester transfer activity in plasma. In hamsters, adipose tissue and heart were found to be the richest sources of both CETP and LPL mRNA; in situ hybridization studies showed that the same cell types (i.e. adipocytes or myocytes) contained CETP and LPL mRNA in these tissues. Isolated adipocytes synthesized active CETP. Dietary studies revealed a complex pattern of response of CETP mRNA levels in different tissues, which showed partial similarity to the changes in LPL mRNA abundance. However, high cholesterol diets resulted in increased CETP mRNA abundance in adipose tissue, heart, and skeletal muscle, without equivalent changes in LPL mRNA. Plasma HDL cholesteryl ester levels showed strong inverse correlations with CETP mRNA abundance in adipose tissue. The results suggest a conserved function of CETP in adipose tissue and heart, such as a co-ordinate action with LPL to enhance HDL turnover. Although there is considerable overlap in the tissue- and cell-specific pattern of CETP and LPL gene expression, dietary studies revealed only limited parallelism in response at the mRNA level. The increase in CETP mRNA in peripheral tissues in response to increased dietary cholesterol suggests that local induction of CETP synthesis may help to recycle cholesterol deposited in these tissues during lipolysis of dietary lipoproteins. |
| Databáze: | OpenAIRE |
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