A dual-emission two-photon fluorescent probe for specific-cysteine imaging in lysosomes and in vivo
| Autor: | Linke Li, Yan Feng, Xiangming Meng, Chuan Sun, Yinliang Huang, Xin Wang, Fang Guoshun, Qifeng Ren, Wan Zhang, Shanqing Li |
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| Rok vydání: | 2019 |
| Předmět: |
Biocompatibility
Carbazole Metals and Alloys 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology Condensed Matter Physics 01 natural sciences Fluorescence 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials chemistry.chemical_compound chemistry Two-photon excitation microscopy In vivo Intramolecular force Materials Chemistry Biophysics lipids (amino acids peptides and proteins) Titration Electrical and Electronic Engineering 0210 nano-technology Instrumentation Cysteine |
| Zdroj: | Sensors and Actuators B: Chemical. 293:247-255 |
| ISSN: | 0925-4005 |
| DOI: | 10.1016/j.snb.2019.04.120 |
| Popis: | Cysteine (Cys) can effectively maintain lysosomes under a highly reductive environment. However, fluorescent probes for specific detection of lysosomal Cys over other biothiols are rare. Probe Lyso-DCHO utilizing a carbazole-based two-dimensional intramolecular charge transfer (ICT) system was designed to detect Cys with a ratiometric signal (119 nm emission shift). The sensing mechanism was fully supported by 1H-NMR titration, ESI-MS and DFT calculations. Lyso-DCHO can be well localized in lysosomes along with good biocompatibility. Furthermore, Lyso-DCHO achieved a dual-channel fluorescence response toward Cys in lysosomes and zebrafishes with high selectivity under the two-photon (TP) excitation. |
| Databáze: | OpenAIRE |
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