Toxic effect of puromycin on erythrocyte membranes which is unrelated to inhibition of protein synthesis
| Autor: | Seymour M. Sabesin, Samir K. Ballas, Edward R. Burka |
|---|---|
| Rok vydání: | 1975 |
| Předmět: |
Lysis
media_common.quotation_subject Immunology Cell Cell Biology Hematology Biology Biochemistry Molecular biology Cell biology Cell membrane chemistry.chemical_compound Membrane medicine.anatomical_structure chemistry Vacuolization Puromycin medicine Protein biosynthesis Internalization media_common |
| Zdroj: | Blood. 45:21-27 |
| ISSN: | 1528-0020 0006-4971 |
| DOI: | 10.1182/blood.v45.1.21.21 |
| Popis: | Exposure of rabbit or human erythrocytes to concentrations of puromycin as low as 7 x 10(-4)M for 2 hr causes damage to the cell membrane, as evidenced by increased susceptibility of the cells to hyposmotic lysis, increased cell rigidity, and ultrastructural changes consistent with severe membrane damage. Puromycin causes a concentration-dependent internalization of the erythrocyte membrane, resulting in vacuolization of the cells, at concentrations between 7 x 10(-4) M and 10(-2) M. Since the erythrocyte does not synthesize protein, the data indicate that puromycin has a direct toxic effect on erythroid cell membranes which is unrelated to its action in inhibiting the synthesis of protein. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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