Optimization of Fecal Calprotectin Assay for Pig Samples
| Autor: | Jaeyoung Heo, Yeon Jae Choi, Paul Bogere |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Gut inflammation
040301 veterinary sciences Chemistry 04 agricultural and veterinary sciences General Medicine 0403 veterinary science 03 medical and health sciences fluids and secretions 0302 clinical medicine Dilution ratio Double check 030212 general & internal medicine Food science Calprotectin Feces Homogenization (biology) |
| Zdroj: | Journal of Agriculture & Life Science. 53:93-104 |
| ISSN: | 2383-8272 1598-5504 |
| Popis: | Fecal calprotectin is a noninvasive marker of gut inflammation and has been widely utilized in human gastrointestinal diagnostics. This marker, however, has not been extensively utilized in porcine samples. The aim of this study was to optimize a protocol for the extraction of porcine fecal calprotectin, and to the best of our knowledge this is the first study to be conducted in this regard. Freshly collected swine fecal samples were used in this study. We determined the variability of three commercial ELISA assays in the recovery of porcine fecal calprotectin. We further studied the effect of dilution factor and roller shaker homogenization on the yield of calprotectin from swine fecal samples. Calprotectin recovery was significantly different(p |
| Databáze: | OpenAIRE |
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