Direct estimation of urine estrone conjugate for a rapid pregnancy diagnosis in sows

Autor: Carlo Tamanini, Mauro Mattioli, Eraldo Seren, R. Gaiani
Rok vydání: 1983
Předmět:
Zdroj: Theriogenology. 19:817-822
ISSN: 0093-691X
DOI: 10.1016/0093-691x(93)90325-y
Popis: A rapid and direct radioimmunoassay of urine estrone conjugate (E 1 C) was developed. Urine samples were taken from Large White sows by inserting sponges in the vagina which were expelled during micturition. Analysis of samples collected daily from 13 sows between 2 and 40 days after service showed that high urine E 1 C levels occurred between day 20 and 30 after insemination in pregnant sows; maximum concentrations were observed on day 25 (79.4 ± 11.7 ng/ml, X ± s.e.m. ). In this period E 1 C levels never exceeded 1 ng/ml in nonpregnant sows. A single urine sample was then taken from 84 sows, 25 days after insemination, to check the accuracy of E 1 C estimation as a test for early pregnancy diagnosis. Seventy-six of the examined animals were correctly diagnosed, giving an overall accuracy of 90.4%. No relationship was found between litter size and urine E 1 C levels.
Databáze: OpenAIRE